5-Amino-1MQ (10 Vial Box)

Compliance & Disclaimer

• For Research Use Only
• Not FDA or EMA approved for clinical application
• Evidence is limited to preclinical studies; safety and efficacy in humans remain unestablished.

5-Amino-1MQ: Mechanistic Overview and Research Context

5-Amino-1-methylquinolinium (5-amino-1MQ) is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that catalyzes the methylation of nicotinamide using S-adenosyl-L-methionine (SAM) as a methyl donor. NNMT activity intersects two critical metabolic pathways: the NAD⁺ salvage pathway and one-carbon methyl-group metabolism. Elevated NNMT expression has been documented in adipose tissue, liver, and stromal compartments during obesity, insulin resistance, and certain cancers. [netnewsledger.com], [frontiersin.org], [link.springer.com]

By competitively inhibiting NNMT, 5-amino-1MQ reduces the formation of 1-methylnicotinamide (1-MNA), thereby preserving nicotinamide for NAD⁺ biosynthesis and conserving methyl donors for epigenetic processes. This biochemical shift increases intracellular NAD⁺ availability, which supports mitochondrial oxidative metabolism and enhances the activity of NAD⁺-dependent enzymes such as sirtuins (e.g., SIRT1), key regulators of energy homeostasis and chromatin remodeling. [fluxlabsusa.com], [nature.com]

Preclinical Evidence

In diet-induced obese (DIO) mouse models, NNMT inhibition via 5-amino-1MQ has been associated with:

• Reduced adiposity and adipocyte size (up to ~30–35% decrease in white adipose tissue mass)
• Improved glucose tolerance and insulin sensitivity
• Elevated tissue NAD⁺ levels and restored methylation potential
• Weight reduction (~5–7% within 10–11 days) without changes in food intake. [peptidesciences.com], [fluxlabsusa.com], [benchchem.com]

Mechanistically, these effects are linked to enhanced mitochondrial respiration, increased fatty acid oxidation, and improved metabolic flexibility. Importantly, NNMT inhibition does not act as a central nervous system stimulant; rather, it exerts its influence at the enzymatic level, making it a valuable research tool for studying metabolic regulation. [fluxlabsusa.com]

Research Applications

Current investigations focus on NNMT inhibition as a strategy to explore:

• Adipose tissue remodeling and energy expenditure
• Epigenetic regulation via SAM/SAH ratio modulation
• NAD⁺-dependent signaling pathways, including sirtuin activation
• Potential roles in metabolic disorders, cancer biology, and aging-related decline. [link.springer.com], [frontiersin.org]

Key References

1. Ulanovskaya OA, et al. NNMT promotes epigenetic remodeling in cancer by consuming methyl donors. Nature Chem Biol. 2017;13(6):646–652. doi:10.1038/nchembio.2361.
2. Sun W-D, et al. Nicotinamide N-methyltransferase: a novel therapeutic target for metabolic syndrome. Front Pharmacol. 2024;15:1410479. doi:10.3389/fphar.2024.1410479. [frontiersin.org]
3. Park J, et al. Exploring NNMT: from metabolic pathways to therapeutic targets. Arch Pharm Res. 2024;47:893–913. doi:10.1007/s12272-024-01519-9. [link.springer.com]
4. FluxLabs Research Review. 5-Amino-1MQ: Targeting Fat Storage and Aging Through NNMT Inhibition. 2025. [fluxlabsusa.com]
5. BenchChem Technical Report. Comparative Analysis of Preclinical NNMT Inhibitors. 2025. [benchchem.com]