Epithalon (10 Vial Box)

Compliance & Disclaimer

• For Research Use Only
• Not FDA or EMA approved for clinical application
• Evidence is limited to preclinical studies; safety and efficacy in humans remain unestablished.

Epithalon is a synthetic tetrapeptide with the sequence Ala‑Glu‑Asp‑Gly (AEDG), originally designed as a defined analogue of Epithalamin, a pineal gland polypeptide complex studied in Russia for geroprotective effects. Research spanning in vitro, in vivo, and limited human contexts has examined Epithalon’s roles in telomere biology, pineal neuroendocrine regulation, antioxidant responses, and immune modulation. [mdpi.com]

Mechanistic Insights

• Telomere Maintenance via Telomerase:
  Multiple lines of evidence indicate Epithalon can upregulate telomerase activity, thereby supporting telomere length maintenance and extending replicative lifespan in cultured human cells; recent work shows dose‑dependent telomere extension via hTERT/telomerase upregulation in normal cells, with ALT pathway activation observed in certain cancer lines (highlighting model‑dependent mechanisms). A 2025 review synthesizes decades of telomere‑targeting data and positions Epithalon as a candidate geroprotective modulator, while emphasizing the need for rigorous clinical validation. [link.springer.com] [mdpi.com]

• Pineal Gland & Melatonin Regulation:
  Epithalon research suggests direct influence on melatonin synthesis and circadian regulation, consistent with pineal peptide biology; however, species/model results vary (e.g., discrepant rat vs. primate findings), underscoring that neuroendocrine effects are context‑dependent and require further controlled trials. [mdpi.com], [alzdiscovery.org]

• Antioxidant/Anti‑mutagenic Actions:
  Reviews catalog antioxidant, neuroprotective, and anti‑mutagenic effects—e.g., normalization of oxidative stress indices, modulation of enzymes (AChE, BuChE), and support of cellular defenses—though exact upstream pathways (e.g., Nrf2‑linked programs) remain under active investigation. [mdpi.com]

Research Applications

• Cellular Aging & Telomere Biology:
  Epithalon serves as a tool to test hypotheses in replicative senescence, telomere dynamics, and healthy cellular lifespan, enabling studies of hTERT expression and telomere length endpoints in normal vs. transformed cell systems. [link.springer.com]

• Circadian/Endocrine Research:
  By probing pineal function and melatonin rhythms, Epithalon is used to explore sleep‑wake regulation and age‑related circadian drift; melatonin’s broader immunomodulatory links provide a rationale for integrative neuroendocrine‑immune investigations (the melatonin literature independently connects circadian integrity with immune homeostasis). [mdpi.com], [mdpi.com]

• Oxidative Stress & Immune Modulation:
  Preclinical datasets report changes in oxidative stress markers and selected cytokines (e.g., IL‑2 mRNA modulation), motivating controlled model systems to delineate redox and immune axes where AEDG might act as a fine‑tuning rather than a gross “boosting” intervention. [mdpi.com]

Important context: The Alzheimer’s Drug Discovery Foundation’s Cognitive Vitality review notes that human evidence is limited, often from single‑center studies, and stresses heterogeneity and translation gaps—especially in neurocognition—warranting caution and rigorous, multi‑site trials before clinical adoption. [alzdiscovery.org]

Key Peer‑Reviewed & Scientific References

1. Araj S.K. et al. Overview of Epitalon—Highly Bioactive Pineal Tetrapeptide with Promising Properties. Int J Mol Sci. 2025;26(6):2691. DOI:10.3390/ijms26062691 — comprehensive mechanistic and pharmacodynamic review (telomerase, melatonin, enzymatic/immune effects). [mdpi.com]
2. Al‑dulaimi S. et al. Epitalon increases telomere length in human cell lines through telomerase upregulation or ALT activity. Biogerontology. 2025;26:178 — quantitative cell‑line study detailing hTERT/telomerase vs. ALT pathways in normal vs. cancer cells. [link.springer.com]
3. ADDF Cognitive Vitality. Epithalamin/Epithalon: Evidence Summary. 2025 — balanced review of preclinical vs. limited clinical data, with discussion of melatonin/circadian and antioxidant findings; calls for larger trials. [alzdiscovery.org]
4. Peptide Initiative. How Epithalon Works – Telomerase & Pineal Mechanisms. 2025 — research overview and mechanistic synthesis (telomere, pineal pathways) for laboratory contexts. [peptideini...iative.com]
5. BioLongevity Labs. Epitalon Peptide Science and Aging Research. 2025 — summary of AEDG origins, model variability, and multi‑pathway aging research orientation. [biolongevitylabs.com]